Plate Sealer/Plate Peeler
Many molecular biology reactions are carried out in 96 or 386-well plates and require the use of a thermocycler or incubator. To prevent the evaporation of reaction contents under the heat of those instruments, a seal must be placed on the labware before heating and removed after heating. Currently, our robotic platforms lack this capability which limits the breadth of biological protocols we are able to perform.
We recently purchased two instruments for the RPL, an Azenta plate sealer and plate peeler. Instrument details are provided below. Students on this project will develop a python API to allow fully open-source and automated control of both instruments.
Instrument Details:
Hudson SciClops Microplate Handler
Related GitHub repo: https://github.com/AD-SDL/hudson_driver
Thermocycler
Our production-ready automated robotic platform does not contain a thermocycler which limits the platform’s capabilities with biological workflows. A thermocycler is needed to enable fully automated DNA Assembly, a workflow that Argonne is very interested in implementing. Students on this project will create a python API and ROS2 layer to interface with Analytic Jena Biometra TRobot IIs. This will enable the use of this thermocycler on our future fully open-source robotic experimentation platforms.
Instrument Details:
Analytik Jena Biometra TRobot II
Related GitHub Repo: https://github.com/AD-SDL/biometra_driver
Mentors: Rafael Vescovi, Doga Ozgulbas, Casey Stone
Students: Sanjiv Parthasarathy, Noah Grom, Amaan Khan, Halona Dantes, Saaya Patel
This project aims to enable computer vision capabilities on a mobile robot in such a way that decisions can be made local to the robot in real time based on video input. This includes design and initial development of a vision API. This could include adding vision to UR5e arm so that QR-type tags could (1) identify fiducial positions to provide a coordinate system transformation to enable accurate interactions with mobile arm and things on static tables, and (2) identify things of interest called out by name in protocol: “beaker 7”, “start button”, “trash bin”